Figure 2.

TetOn-induced expression of PIKfyve^K1831E but not that of PIKfyve^WT is associated with appearance of multiple cytoplasmic vacuoles. Indicated HEK293 cell lines, stably expressing HA-PIKfyve^WT (clone 9), HAPIKfyve^K1831E (clone 5), or control TetOn were seeded in the presence or absence of doxycycline as described under MATERIALS AND METHODS. Eighteen hours postinduction of protein expression, cells were observed live with a TE200 microscope (Nikon), and images were captured by a SPOT RT Slider camera. Bar, 10 μm.