TABLE 1.
Affinity (Kd) of Polη, Polκ, and Polι peptides interacting with PCNA Boldface and italic characters indicate PIP-box residues and notable alterations, respectively. Asterisks indicate the C termini of the corresponding proteins. ND, not detected.
| Peptide (region) | Property | Sequence | Kd |
|---|---|---|---|
| μm | |||
| Polη-(694–713) | Wild type | CKRPRPEGMQTLESFFKPLTH* | 0.40 |
| M701Q | CKRPRPEGQQTLESFFKPLTH* | 0.10 | |
| F707A/F708A | CKRPRPEGMQTLESAAKPLTH* | ND | |
| ΔPLTH | CKRPRPEGMQTLESFFK–– | ND | |
| Polκ-(851–870) | Wild type | CSTSKKIKPNNPKHTLDIFFK* | ND |
| Polκ-(856–870) | Wild type | CIKPNNPKHTLDIFFK* | ND |
| K862Q | CIKPNNPQHTLDIFFK* | ND | |
| +PLTH | CIKPNNPKHTLDIFFKPLTH | 4.9 | |
| Polι-(419–434) | Wild type | CAKKGLIDYYLMPSLST | 0.39 |
| Y426F/Y427F | CAKKGLIDFFLMPSLST | ND | |
| Y426A/Y427A | CAKKGLIDAALMPSLST | ND | |
| Y426F | CAKKGLIDFYLMPSLST | 1.5 | |
| Y427F | CAKKGLIDYFLMPSLST | ND | |
| L428A | CAKKGLIDYYAMPSLST | 0.67 |