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. 2009 Apr 17;284(16):10571–10582. doi: 10.1074/jbc.M900752200

FIGURE 4.

FIGURE 4.

IP6K1 over-expression rescues TNP inhibition of InsP7 synthesis. A, HPLC of InsP6 and InsP7 from HeLa cells overexpressing GFP-IP6K1 labeled with [3H]inositol. Cells were stimulated with DMSO (filled circles) or TNP at 100 nm (open circles); 1 μm (open triangles), and 10 μm (closed triangles) for 2 h at 37 °C. The soluble inositol phosphates were extracted and separated as explained under “Experimental Procedures.” B, decrease in InsP7 from HeLa cells overexpressing GFP (filled circles) or GFP-IP6K1 (open circles) as a function of TNP concentration. Data are the average of triplicate experiments, and error bars represent the S.D. of the data. Curve fitting to the InsP7 data was done using the Sigma Plot software, and IC50 calculations were carried out from the equation used to fit the curve.