Skip to main content
. 2009 Apr 17;284(16):10583–10592. doi: 10.1074/jbc.M900847200

FIGURE 1.

FIGURE 1.

Effects of overexpression of ARFRP1(T31N)-HA or ARL1(T31N) on the localization of Golgi proteins. HeLa cells were transfected with an expression vector for C-terminally HA-tagged ARFRP1(T31N) (A) or untagged ARL1(T31N) (B–D) and doubly stained with antibodies to HA and ARL1 (A and A′), golgin-245 and ARFRP1 (B and B′), ARL1 and GM130 (C and C′), or ARL1 and CI-MPR (D and D′). Cells overexpressing ARFRP1(T31N)-HA or ARL1(T31N) are indicated by asterisks; nontransfected cells are indicated by crosses. In B and B′, cells overexpressing ARL1(T31N) were indirectly identified by redistribution of golgin-245, because of antibody restriction.