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Histological features of the ectopically formed bone are comparable between wild-type and ANA-deficient mice. Ectopically formed ossicles (bone) contain woven bone (hematoxylin and eosin stain; A and B). tartrate-resistant acid phosphatase-positive multinucleated cells were observed in the ossicle (C–F) (scale bars: A–D, 200 μm; E and F, 50 μm). These morphological structures of bone and cells were similar between the two genotypes.
