FIGURE 2.

FACS analysis during MCE of adipocyte differentiation. Cells were induced to differentiate in the presence or absence of H₂O₂, genistein (50 μm), or resveratrol (50 μm), and at the times indicated, cells were trypsinized and fixed. Changes in DNA content were analyzed by FACS using a FACSCalibur flow cytometry system, and data were analyzed using CellQuest software. FACS analysis was performed during 0–72 h of differentiation induction (A) and after 24 h of induction (B). C, cells were differentiated with the standard protocol for 16 h, after which resveratrol (50 μm) was added to the medium. FACS analysis was done at 24 h after induction of differentiation.