FIGURE 4.

Effects of ROS on intracellular localization of C/EBPβ during MCE. 3T3-L1 preadipocytes were induced to differentiate with the standard protocol in the presence or absence of H₂O₂ (100 μm), resveratrol (50 μm), or N-acetyl cysteine (10 mm), as indicated. Cells were fixed and subjected to immunofluorescence analysis with antibody against C/EBPβ and 4′,6-diamidino-2-phenylindole. Fluorescence images were obtained by confocal microscopy. Resveratrol (A) and N-acetyl cysteine (B) prevented the characteristic punctate pattern at 16 h of induction and also rapidly dispersed the centromeric localization after short term treatment (14–18 h of induction). C, H₂O₂ caused the early appearance of a punctate pattern in immunofluorescent stainings.