TABLE 1.

Primers used for site-directed mutagenesis

Mutant	DNA template	Primer
F396A	ChiA wild type	Forward	5′-GACTTCTATGGCGCCGCCGATCTGAAGAAC-3′
		Reverse	5′-GTTCTTCAGATCGGCGGCGCCATAGAAGTC-3′
W275A	ChiA wild type	Forward	5′-GATCGGCGGCGCGACGCTGTCCG-3′
		Reverse	5′-CGGACAGCGTCGCGCCGCCGATC-3′
W167A	ChiA wild type	Forward	5′-CGTCGAGGCGGGCGTTTAC-3′
		Reverse	5′-GTAAACGCCCGCCTCGACG-3′
W167A/W275A	W275A mutant	Forward	5′-CGTCGAGGCGGGCGTTTAC-3′
		Reverse	5′-GTAAACGCCCGCCTCGACG-3′