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Efficiency of hydrolysis of the substrates GluNA, LeuNA and LysNA by the recombinant APAs. Recombinant wild type and mutated APAs hydrolysis efficiencies were evaluated for GluNA, LeuNA and LysNA in the absence (A) or the presence (B) of 4 mm Ca2+. The data shown are the means ± S.E. of three to seven experiments performed in duplicate.
