. 2009 Apr 17;284(16):10618–10626. doi: 10.1074/jbc.M806783200

TABLE 2.

Kinetic parameters for wild type and mutated APAs K_m and k_cat values mean ± S.E. from three to nine separate experiments with duplicate determinations.

Recombinant APAs	No Ca²⁺			4 mm Ca²⁺
Recombinant APAs	*K_m*	k_cat	k_cat/K_m	*K_m*	k_cat	k_cat/K_m
	μm	s^–1	mm^–1·s^–1	μm	s^–1	mm^–1·s^–1
Wild type	1481 ± 60	292 ± 10	197	198 ± 19^a	256 ± 5	1319
Ser-348	2034 ± 391^a	640 ± 121^b	314^a	159 ± 17^a	575 ± 25^b	3616^b
Asp-348	1136 ± 48^a	92 ± 3^c	81^b	258 ± 25^a	40 ± 2^c	155^d
Tyr-348	1919 ± 102^a	28 ± 4^d	15^c	282 ± 13^a	167 ± 6^a	596^b

Values not significant when compared with the corresponding wild type values

p < 0.05

p < 0.01

p < 0.001