Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Apr 17;284(16):10650–10658. doi: 10.1074/jbc.M809094200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 5. — Cdt holotoxin containing a CRAC mutation (CdtABC^Y71P) exhibits reduced ability to associate with cholesterol. Panel A shows the results of the FRET analysis of the ability of CdtABC^WT versus that of CdtABC^Y71P to bind to LUVs containing 10–40 mol % cholesterol. Results are plotted as the percentage of energy transfer versus cholesterol concentration and represent the mean ± S.D. of three experiments. Panel B, SPR analysis of the ability of immobilized CdtABC^WT (broken line) and CdtABC^Y71P (solid line) to bind LUVs containing 20% cholesterol; results are representative of three experiments. Results are statistically significant (p < 0.035) for the differences between CdtABC^wt and CdtABC^Y71P at all concentrations of cholesterol.