FIGURE 7.

CdtABC^Y71P exhibits reduced ability to induce cell cycle arrest. Jurkat cells were incubated with medium alone (panel A), 50 pg/ml CdtABC^WT (panel B), or 50 pg/ml CdtABC^Y71P (panel C) for 18 h, stained with propidium iodide, and analyzed for cell cycle distribution by flow cytometry as described under “Experimental Procedures.” Numbers represent the percentage of cells in the G₀/G₁, S, and G₂/M phases of the cell cycle. Results are representative of three experiments; 15,000 cells were analyzed for each sample. PI, propidium iodide.