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Kinetic parameters for M. barkeri tRNASer aminoacylation with wild-type and mutant mMbSerRS enzymes
| mMbSerRS | Km | kcat | kcat/Km | kcat/Km (rel) |
|---|---|---|---|---|
| μm | s–1 | mm–1 s–1 | ||
| WT | 2.6 ± 0.5 | 0.45 ± 0.04 | 173 | 1 |
| R147A | 14.6 ± 0.7 | 0.15 ± 0.01 | 10.6 | 0.06 |
| E273A | 4.2 ± 0.6 | 0.55 ± 0.10 | 130 | 0.75 |
| E273A/D277A/K280A | 4.7 ± 0.6 | 1.4 ± 0.2 | 290 | 1.68 |
| K164A | 2.4 ± 0.4 | 0.0087 ± 0.0009 | 3.6 | 0.02 |
