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. 2009 Apr 17;284(16):10728–10736. doi: 10.1074/jbc.M900166200

FIGURE 1.

FIGURE 1.

8-pCPT-2′-O-Me-cAMP-AM activates the cAMP reporter Epac1-camps. A, expression of a fusion protein of Epac1 and EGFP in stably transfected INS-1 cells (calibration bar, 0.8 μm). B, stable expression of Epac1-camps in INS-1 cells (calibration bar, 3 μm). C, FRET-based live cell imaging of Epac1-camps in INS-1 cells exposed to 8-pCPT-2′-O-Me-cAMP-AM (20 μm; arrows indicate duration of exposure). D, single cell population study summarizing the action of 8-pCPT-2′-O-Me-cAMP-AM (ESCA-AM; 20 μm) to activate Epac1-camps in INS-1 cells. Note that both the non-AM-ester of 8-pCPT-2′-O-Me-cAMP (ESCA, no AM;20 μm) and acetoxymethyl ester phosphate (Phosphate-AM3; 6.6 μm) were without significant effect. All solutions were administered in SES containing 0.1% dimethyl sulfoxide. Values above each histogram bar indicate the numbers of cells (n values) assayed for each experimental condition. For panels C and D, the experiment was repeated 3 times with similar results.