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. 2009 Apr 17;284(16):10728–10736. doi: 10.1074/jbc.M900166200

FIGURE 2.

FIGURE 2.

High throughput assay for activation of Epac1-camps by cAMP analogs. A, concentration-response relationship for the activation of Epac1-camps by 0.3–3.0 μm 8-pCPT-2′-O-Me-cAMP-AM (ESCA-AM). The vehicle solution was comprised of SES containing 0.1% dimethyl sulfoxide. A vertical arrow indicates the time at which the test solutions were injected. An increase of 485/535 nm emission ratio signifies the activation of Epac1-camps. B, differential potencies of 8-pCPT-2′-O-Me-cAMP-AM (ESCA-AM, blue line and symbols) and 8-pCPT-2′-O-Me-cAMP (ESCA-No-AM, red line and symbols) to activate Epac1-camps. For panels A and B, each data point corresponds to the mean of 24–36 wells, and each experiment was repeated 3 times with similar results.