High throughput assay for activation of Epac1-camps by cAMP analogs.
A, concentration-response relationship for the activation of
Epac1-camps by 0.3–3.0 μm
8-pCPT-2′-O-Me-cAMP-AM (ESCA-AM). The vehicle solution
was comprised of SES containing 0.1% dimethyl sulfoxide. A vertical
arrow indicates the time at which the test solutions were injected. An
increase of 485/535 nm emission ratio signifies the activation of Epac1-camps.
B, differential potencies of 8-pCPT-2′-O-Me-cAMP-AM
(ESCA-AM, blue line and symbols) and
8-pCPT-2′-O-Me-cAMP (ESCA-No-AM, red line and
symbols) to activate Epac1-camps. For panels A and
B, each data point corresponds to the mean of 24–36 wells, and
each experiment was repeated 3 times with similar results.