TABLE 1.
Regulators of TAF1-3 and TAF1-4 splicing
|
Genea
|
CG number (isoform)
|
Target
nucleotidesb
|
-Fold
increasec
|
Additive with
CPTd
|
|
|---|---|---|---|---|---|
| TAF1–3 | TAF1–4 | ||||
| Tra2 | CD10128 (Tra2-RD) | 503–812 | 3.7 | 2.7 | No |
| 140–472 | 2.7 | 2.3 | NDe | ||
| 480–806 | 2.7 | 2.2 | ND | ||
| Rox8/dTIAR | CG5422 (Rox8-RD) | 1575–2005 | 2.2 | 2.7 | No |
| 980–1612 | 2.3 | 2.4 | ND | ||
| 1683–2377 | 2.3 | 2.3 | ND | ||
| Tia1-like | CG34362 | 1559–1953 | 3.5 | 3.9 | No |
| (Tia1-like-RA) | 653–1405 | 3.5 | 3.6 | ND | |
| 1455–2155 | 3.9 | 4.1 | ND | ||
| Crn | CG3193 (Crn-RA) | 119–2227 | 2.8 | 2.9 | No |
| 289–1138 | 2.6 | 2.0 | ND | ||
| 1245–2107 | 2.4 | 2.2 | ND | ||
| PUF60/hfp | CG12085 (PUF60-RA) | 921–1170 | 3.5 | 2.8 | No |
| 1291–2093 | 2.8 | 2.6 | ND | ||
| U2AF38 | CG3582 (U2AF38-RA) | 196–990 | 2.5 | 3.0 | No |
| 196–592 | 3.5 | 3.0 | ND | ||
| 622–988 | 2.0 | 2.0 | ND | ||
| KEP1 | CG3584 | 268–720 | 2.0 | 2.3 | Yes |
| U1C | CG5454 | 109–546 | 1.8 | 2.5 | Yes |
| Brr2 | CG5931 | 5791–6172 | 3.3 | 3.0 | Yes |
| UAP56/Hel25E | CG7269 | 411–863 | 2.3 | 1.9 | Yes |
| eIF-4A | CG9075 | 831–1163 | 2.5 | 1.8 | Yes |
| Caper | CG11266 | 1120–1459 | 3.0 | 3.3 | Yes |
| Prp39 | CG1646 | 1076–1562 | 2.5 | 2.0 | Yes |
| LSM4 | CG31990 | 1304–1516 | 2.5 | 2.5 | Yes |
| Prp16 | CG32604 | 432–786 | 1.9 | 2.8 | Yes |
a
Naming of Tia1-like and Prp16 is based on sequence similarity
b
Nucleotides complementary to the dsRNA
c
Average -fold increase relative to mock RNAi-treated cells (n = 3)
d
Additivity of RNAi and CPT effects on TAF1-3 and TAF1-4 splicing
e
ND, not determined