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Inhibition of membrane ATPase activity from mutant and wild type ATP synthase enzymes by DCCD. The membranes were preincubated for 16 h at room temperature with varied concentrations of DCCD indicated in the figure. Then 1 ml of ATPase assay buffer was added to determine the activity. •, wild type; ○, αS347A; □, αS347Q; ▵, αG351Q. All of the data points are means of at least quadruplicate experiments. The variation was ±10% between different experiments.
