TABLE 1.
Mutationa | Growth on succinateb | Growth yield in limiting glucose | ATPase activityc |
---|---|---|---|
% | μmol/min/mg | ||
Wild type | ++++ | 100 | 28 |
Null | – | 46 | 0 |
βY331W | ++++ | 95 | 26 |
αS347Q | ± | 55 | 0.20 |
αS347A | + | 65 | 2.18 |
αG351Q | ± | 54 | 0.25 |
Wild type, pBWU13.4/DK8; Null, pUC118/DK8, αS347Q/DK8, αG351Q/DK8, and αS347A/AN888. Both αS347Q and αG351Q mutants were expressed with the βY331W mutation also present, which does not significantly affect growth. The data are the means of four to six experiments each
Growth on succinate plates after 3 days estimated by eye. ++++, heavy growth; +, partial growth; ±, very light growth; –, no growth
ATPase activity was measured at 37°C and expressed as μmol of ATP hydrolyzed/min/mg of membrane protein. Each individual experimental point is itself the mean of duplicate assay tubes. The data are derived from two separate membrane preparations. The results from separate membrane preparations were in excellent agreement within ± 10%