FIGURE 6.

Expression of FOXC2-VP16 or FOXC2 impairs induction of C/EBPβ during adipocyte differentiation or in response to cAMP. A and B, FOXC2-VP16 and FOXC2-infected 3T3-L1 cells, respectively, were induced to differentiate in presence of MDI. At the indicated times, cells were lysed, and C/EBPβ (LAP1, LAP2, and LIP) expression was determined by immunoblot analysis. C, at confluence, control or FOXC2-VP16 cells were treated for four h with 10% calf serum (CS), MDI, isobutylmethylxanthine (IBMX), or increasing concentrations of 8-bromo-cAMP in Me₂SO as indicated. The cells were lysed, and expression of C/EBPβ proteins was evaluated. The experiments are representative of three independent experiments.