Role for Hey1 in suppression of adipogenesis by FOXC2. A,
3T3-L1 preadipocytes were infected with a retrovirus vector alone (pBabe or
pLNCX2) or retroviruses carrying the genes for FOXC2-VP16, FOXC2-Eng, or FOXC2
as indicated. Two days post-confluence, the cells were induced to
differentiate with MDI. RNA was purified at the indicated times and expression
of Hey1 analyzed by quantitative PCR. B, 3T3-L1 preadipocytes were
infected with a retrovirus vector alone (pBabe) or a vector carrying the gene
for Hey1. Two days after confluence, the cells were induced to differentiate
in presence of MDI or MDI+T. The cells were stained with Oil Red-O on day 14.
C, on the indicated days of differentiation, the cells were lysed,
and expression of C/EBPβ, C/EBPα, PPARγ, and FABP4 was
analyzed by immunoblot analysis. D, RNA was purified from cells
described in C and analyzed by quantitative PCR. The experiments are
representative of at least two independent experiments.