FIGURE 2.

Viral replication is required for induction of type I IFN production in fibroblasts and full activation of dendritic cells. MEFs (A), BM-DCs (B), and BC-1 DCs (C-E) were infected with live or UV-inactivated SeV at MOI 1. Cell culture supernatants were collected 18 h after infection, and cytokine levels were determined. In C, the cells received infectious virus (MOI 1) or a similar dose of virus that had been UV-treated for the indicated times (1-7 min). The data are shown as means of triplicates ± S.D. UT, untreated cells.