FIGURE 5.

Defective respiration of Δisc1 in the post-diauxic phase. A, induction ratio of carbon source metabolism pathway genes across diauxic shift in WT and Δisc1. Carbon source metabolism pathway was modified from a review by Schuller (45). Red boxes indicate the genes more induced across the diauxic shift in Δisc1 relative to WT according to the ratio of WT (24 h)/WT (4 h)/Δisc1 (24 h)/Δisc1 (4 h). Blue boxes indicate the genes less induced across the diauxic shift in Δisc1 relative to WT. Thickness of the boxes indicates the fold change as shown in the figure. The results are representatives of at least two independent experiments. B, Venn diagram of the number of whole genome (blue), diauxic up-regulated (orange), glucose-repressed (green), and Isc1p-dependent diauxic up-regulated (purple) genes. A cohort of glucose-repressed genes was extracted from glucose pulse experiment (33). C, growth of WT and Δisc1 in ethanol-containing media. WT and Δisc1 cells in the post-diauxic phase were inoculated to fresh YPethanol and incubated at 30 °C for the indicated times. D, oxygen consumption rate of whole cells (WT and Δisc1) in the post-diauxic phase was measured during incubation with 2% ethanol. The results are represented as mean ± S.E. of at least two independent experiments.