FIGURE 7.

Suppression of induction of ADH2 and CTA1 in respiratory-deficient petite cells during the diauxic shift and upon transfer to nonfermentable medium. A, growth curve of grande and petite cells in YPD medium as monitored by glucose concentration and A₆₆₀. The expression of ADH2 (B) and CTA1 (C) was quantitated by real time RT-PCR at the indicated times. D and E, logarithmically growing cells were transferred from YPD to YPethanol and incubated for 2 h, and the expression of ADH2 (D) and CTA1 (E) was quantitated by real time RT-PCR. The results are represented as means ± S.E. of relative expression of at least three experiments. F, Venn diagram of the number of whole genome (blue), diauxic up-regulated (orange), glucose-repressed (green), and mitochondrial function-dependent diauxic up-regulated (brown) genes. A cohort of glucose-repressed genes was extracted from glucose pulse experiment (33).