TABLE 1.
Transcriptional activities of the different Krox20 mutants
The table summarizes the transcriptional activities of the various Krox20 mutants tested on five different target genes (Krox20, Nab, EphA4, follistatin, and Hoxb1) and evaluated as defined under “Experimental Procedures.” +, activation; +/0, very weak activation; −, repression; 0, no activity. ND, not determined. For each construct, the ratio in parenthesis indicates the number of embryos showing the indicated transcriptional pattern over the total number of observed embryos.
| Krox20 | Krox20 Δ1-50 | Krox20 Δ1-81 | Krox20 Δ1-124 | Krox20 Δ1-158 | Krox20 Δ1-190 | Krox20 Δ1-216 | Krox20 Δ1-235 | Krox20 Δ1-258 | Krox20 Δ1-309 | Krox20 Δ159-189 | Krox20 I268F | VP16 Krox20 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Krox20 | + (27/32) | + (5/5) | + (4/4) | +/0 (11/11) | +/0 (10/10) | − (14/14) | ND | ND | − (6/6) | − (4/5) | + (3/4) | + (7/7) | 0 (6/6) |
| Nab | + (21/22) | ND | + (4/4) | + (4/5) | + (3/4) | 0 (17/17) | ND | ND | 0 (4/4) | 0 (4/4) | + (5/6) | + (16/17) | + (6/6) |
| KphA4 | + (36/38) | ND | + (4/4) | + (4/4) | + (7/7) | + (18/20) | + (10/12) | +/0 (7/7) | +/0 (8/8) | +/0 (8/8) | + (8/10) | + (12/13) | 0 (14/14) |
| follistatin | − (7/10) | ND | ND | − (3/4) | ND | − (5/12) | − (4/5) | 0 (5/5) | 0 (8/8) | 0 (5/5) | ND | ND | ND |
| Hoxb1 | − (20/26) | ND | ND | ND | ND | − (6/6) | ND | ND | − (3/4) | − (4/7) | ND | − (3/4) | ND |