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Immunofluorescent detection of the cp-2 fragment by a specific antibody to the neo-epitope Arg167 of Htt. HEK293 (A) or HT22 (B) cells were transfected with indicated Htt constructs. Epitope between residues 115-129 of Htt is shown in green (FITC); Arg167 neo-epitope is shown in red (Cy 3); the nuclear staining (4′,6-diamidino-2-phenylindole) is shown in blue. Yellow staining in merged images (marked by arrows) demonstrates the presence of cp-2.
