FIGURE 9.

Alteration of cp-2 cleavage site results in a decrease in toxicity of Htt protein in neuronal cells. A, cell viability (measured by luciferase assay, see “Experimental Procedures”) of HT22 cells, expressing the indicated Htt constructs co-transfected with the luciferase construct (n = 3, ^**, p = 0.018 N511-52Q versus N511-52Q-Δ167-170). One representative experiment of three for each set of constructs is shown. B, the expression levels of the indicated constructs were verified by Western blotting with an antibody to FLAG.