FIGURE 1.

Inhibition of Wnt3a-dependent signaling by recombinant sclerostin produced in mammalian cells and E. coli. MC3T3-E1 cells stably transfected with a Super-TOPFlash reporter construct were treated with Wnt3a (50 ng/ml) and purified human recombinant sclerostin expressed either in mammalian cells or E. coli. The data shown illustrate the comparable inhibition of Wnt-dependent signaling obtained with either mammalian cell-derived sclerostin (black bars) or E. coli-derived ¹⁵N-labeled sclerostin (white bars).