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SED1 and AFR1 induction by zymolyase in mutants of the CWI, HOG, mating, and filamentation pathways. Expression of SED1-LacZ and AFR1-LacZ was determined in wild type BY4741 and sho1Δ, ste11Δ, ste50Δ, ssk1Δ, pbs2Δ, hog1Δ, wsc1Δ, mid2Δ, bck1Δ, slt2Δ, rlm1Δ, fus3Δ, and kss1Δ mutant cells growing in the absence (white bars) or presence of 0.8 unit/ml zymolyase (black bars). The results are represented as the means with SDs derived from three independent experiments.
