FIGURE 4.

Ron knockdown-sensitized HCT116 cells to GFDS-induced apoptosis and impaired their motility. Ron knockdown clones and control cells were grown to 80-90% confluence and the cells were then deprived of growth factors for 5 days. A, DNA fragmentation assays were performed to determine apoptosis as described under “Experimental Procedures.” Error bars indicate S.E. B, cells were harvested and Western blot analyses were performed with Ron or caspase 3 antibody. Actin was used as a loading control. C, Ron knockdown clones and the control cells were grown to confluence. Motility was examined in wound healing assays as described under “Experimental Procedures.” D, Ron knockdown clones and the control cells were starved in growth factor-deprived medium overnight. They were then plated in the transwells and transwell assays were performed as described under “Experimental Procedures.”