FIGURE 5.

Knockdown of Ron kinase inhibited the PI 3-kinase pathway. Ron knockdown clones and control cells were grown to 80-90% confluence and the cells were then deprived of growth factors for 5 days. A, cell lysates were harvested and subjected to PI 3-kinase assays as described under “Experimental Procedures.” B, cell lysates harvested in A were subjected to Western blot analysis with pAKT (S473) or AKT antibody. Actin was used as a loading control. C, cell lysates from A were subjected to Western blot (WB) analyses with a p85 antibody or immunoprecipitated (IP) with a Ron antibody followed by Western blot analyses with a p85 antibody.