FIGURE 4.

Cellular actin organization and genetic interactions of srv2 mutants. A, wild type SRV2 and srv2 mutant strains were grown to log phase, fixed, and stained with Alexa488-phalloidin to visualize filamentous actin. B and C, haploid yeast strains carrying integrated srv2 alleles were crossed to haploid pfy1-4 (B) and cof1-19 (C) strains. Diploids were sporulated and tetrads dissected (minimum 20 tetrads, 80 spores), and all haploid progeny were compared for cell growth on YPD plates at 25, 30, 34, and 37 °C. For each cross, we determined the percentage of haploid progeny that grew normally at all temperatures compared with a wild type strain (healthy), exhibited impaired growth at 37 °C (TS), exhibited impaired growth at all temperatures (sick), or were dead at 25 °C (dead).