TABLE 1.
Hydrodynamic properties of purified Srv2 polypeptides
| Construct name | Residues | Molecular massa | Sedimentation coefficientb | Stokes radiusc | Native massd | Oligomerization statee |
|---|---|---|---|---|---|---|
| kDa | S | nm | kDa | |||
| Srv2:actin | 1-526 | 100.8 | 16.1 | 9.9 | 660 | 6.5 |
| C-Srv2 | 253-526 | 30.3 | 2.4 | 5.8 | 57 | 1.9 |
| Srv2ΔCC | 51-526 | 53.0 | 3.4 | 6.7 | 93 | 1.8 |
| Srv2-90 | 1-526 | 58.6 | 9.8 | |||
| Srv2-91 | 1-526 | 58.5 | 9.7 | |||
| Srv2-92 | 1-526 | 58.6 | 9.8 | |||
| Srv2-93 | 1-526 | 58.7 | 9.8 | |||
| Srv2-94 | 1-526 | 58.7 | 9.9 |
a
Molecular mass of a monomeric polypeptide is shown, inclusive of its His6 tag.
b
Data were determined by sucrose gradient sedimentation velocity analysis.
c
Data were determined by gel filtration.
d
Data were calculated from Stokes radius and S value (see “Experimental Procedures”).
e
Ratio of native molecular mass to monomeric molecular mass is shown.