FIGURE 1.

A, cell aggregation assay. Human breast epithelial cells with varying Wnt-5a protein expression levels of HB2 Wnt-5a^low and HB2 Wnt-5a^high cells were treated with Versene, resuspended in Ca²⁺ high as compared with Ca²⁺ low medium and allowed to form aggregates for 1 h upon slow rocking. HB2 Wnt-5a^high cells formed larger cell-cell clusters than HB2 Wnt-5a^low cells. The levels of propidium iodide staining were equal. The diagram represents the fold-increase in number of aggregates formed per approximately 1500 cells in HB2 Wnt-5a^high compared with HB2 Wnt-5a^low cells. Error bars indicate S.E., n = 7, ^***, p < 0.001. B, immunofluorescence (IF) studies of β-catenin membrane localization in human breast epithelial cells with varying Wnt-5a protein expression levels or treated with recombinant Wnt-5a. HB2 Wnt-5a^low, HB2 Wnt-5a^low treated with recombinant Wnt-5a and HB2 Wnt-5a^high (upper); MCF-7, MCF-7 and treated with recombinant Wnt-5a and MCF-7 Wnt-5a^high (lower)(n = 5).