Figure 1.

Inhibition of M. tuberculosis Erdman glutamine synthetase activity by antisense PS-ODNs. Duplicate M. tuberculosis Erdman cultures were grown for 2 weeks in 1–2 ml of 7H9 medium alone and thereafter in the absence or presence of antisense PS-ODNs, individually or combined, at concentrations of 10 μM. At each time point indicated, cultures were harvested and analyzed for glutamine synthetase activity by the transfer assay as described (2). All data points had a SD of ≤10%, even when different PS-ODN preparations were used.