Figure 3.

Inhibition of cell proliferation of M. tuberculosis Erdman and M. smegmatis 1–2c wild type and recombinant strain by antisense PS-ODNs. Duplicate bacterial cultures were grown for 6 weeks (M. tuberculosis) or 6 days (M. smegmatis) in 1–2 ml of 7H9 medium in the presence of the antisense PS-ODNs, individually or combined, at concentrations of 10 μM. At each time point, cultures were harvested, washed, serially diluted, and plated on 7H11 agar medium. Viable bacteria were enumerated after an incubation period of 2 weeks (M. tuberculosis) or 3 days (M. smegmatis). SDs varied between 15 and 20%.