Figure 4.

Inhibition of cell proliferation of M. tuberculosis by antisense PS-ODNs in the presence of various concentrations of ethambutol or polymyxin B nonapeptide. Duplicate bacterial cultures were grown for 6 weeks in 1–2 ml of 7H9 medium in the presence of the antisense PS-ODNs, individually or combined, at concentrations of 10 μM and 0, 0.1, 0.25, or 0.5 μg/ml ethambutol (EMB) and/or polymyxin B nonapeptide (PMBN) to “soften” the cell wall. At each time point, cultures were harvested, washed, serially diluted, plated on 7H11 agar medium, and cfu enumerated after 2 weeks. SDs varied between 15 and 20%. Values for single PS-ODNs were combined to yield one dashed line; the range of values for single PS-ODNs is indicated by the broad, solid, vertical bar.