Figure 2.
The unique function of Tor2 is required for its role in receptor internalization. Internalization of 35S-α-factor was measured at 30°C after growth of cells in YPUAD at 24°C. (A) Wild-type (LHY2632, ♦), rom1Δ (LHY3741, ▴), rom2Δ (LHY3742, ▪), and tus1Δ (LHY3749, ⋄). (B) The same strains assayed in C were tested for growth on YPUAD or YPUAD + 6 mM caffeine plates at 30°C. (C) Wild-type (LHY3877, ♦), tor2G2128R (LHY3878, ▪), sac7Δ (LHY3879, Δ), and tor2G2128R sac7Δ (LHY3880, ⋄). (D) Wild-type (LHY3865; ⋄, ♦) and tor2G2128R (LHY3863; □, ▪) cells were assayed either in YPUAD (open symbol) or YPUAD + 0.005% SDS (closed symbol).