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Ypk1 regulates receptor internalization through Rom2-Rho1. (A) Wild-type (LHY4388), ypk1Δ (LHY4387), sac7Δ (LHY4389), and ypk1Δ sac7Δ (LHY4390) cells were grown on YPUAD or YPUAD + 6 mM caffeine plates at 30°C. (B) The same strains were assayed for internalization of 35S-α-factor at 30°C after growth of cells in YPUAD at 24°C. Wild type (♦), ypk1Δ (▪), sac7Δ (Δ), and ypk1Δ sac7Δ (⋄).
