Figure 5.

Fks1 is a Rho1 effector required for internalization. Internalization of ³⁵S-α-factor was measured at the indicated temperature after growth of cells in YPUAD at 24°C. (A) Wild-type (LHY3604, ♦), rho1-2 (LHY3602, □), and rho1-11 (LHY3603, ▵) cells were assayed at 37°C. (B) Wild-type (LHY4515, ♦), fks1Δ (LHY4477, ▴), and fks2Δ (LHY4514, ▪) cells were assayed at 30°C. (C) Wild-type (LHY2632, ♦), skn7Δ (LHY3746, ⋄), and bni1Δ (LHY3421, X) cells were assayed at 30°C. (D) Wild-type (LHY4172, ♦), bnr1Δ bni1^ts (LHY4171, ▪), pkc1^ts (LHY2817, □), and sec3^ts (LHY3745, ▵) cells were assayed at 37°C.