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. Author manuscript; available in PMC: 2010 Mar 5.
Published in final edited form as: Oncogene. 2009 Jan 12;28(9):1197–1205. doi: 10.1038/onc.2008.465

Figure 6.

Figure 6

Anchorage-independent growth assays of mouse embryonic fibroblasts (MEFs). MEFs with the three genotypes were seeded in soft agar and formation of colonies determined 3 weeks later. A typical photomicrograph is shown for Klf4+/+ (a), Klf4+/− (b) and Klf4−/− (c) MEFs. Note that Klf4+/+ and Klf4+/− cells typically grew to a four-cell stage as shown in the figure but failed to form any colonies. (d) Quantification of the number of colonies formed in soft agar. Shown are the average numbers of colonies per 10-cm plate. N = 3; *P<0.01 compared to Klf4+/+ MEFs.