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. Author manuscript; available in PMC: 2010 Apr 25.
Published in final edited form as: Virology. 2009 Feb 27;387(1):16–28. doi: 10.1016/j.virol.2009.01.041

Figure 2.

Figure 2

(A) Detection of Mamu-A*01 DNA in tail samples from 13 Tg mice by PCR. Cont symbolizes control transgene Mamu-A*01/Kb plasmid DNA template. M represents 1 kb DNA ladder molecular size marker. (B) Detection of cell surface expression of Mamu-A*01/Kb MHC antigen in spleen cells. Splenocytes from DKO (left) mice and Mamu-A*01/Kb Tg (right) mice were stained with FITC-conjugated MAb W6/32 followed by flow cytometry. Splenocytes were also treated with 15 ng/ml IFN-γ for 16 hrs to enhance MHC antigen expression levels.