Table 2.
Nomenclature, molecular weight and amino acid sequence of synthetic minimal CTL and TH epitopes and epitope fusion peptides.
Compound | Name | * MW | Sequence |
---|---|---|---|
Pan DR binding epitope (Alexander et al., | PADRE | 1.354 | AK# XVAAWTLKAAA |
PADRE:SIVGag181–189 fusion | PADRE:CM9 | 2.419 | AK# XVAAWTLKAAACTPYDINQM |
PADRE:SIVGag149–157 fusion | PADRE:LW9 | 2.393 | AK# XVAAWTLKAAALSPRTLNAW |
PADRE:SIVPol961–969 fusion | PADRE:MI8 | 2.266 | AK# XVAAWTLKAAAMTPAERLI |
CpG-ODN #7909 (Hartmann et al., 2000) | CpG-Human | 7.900 | 5′ TCGTCGTTTTGTCGTTTTGTCGTT 3′ |
The sequences are shown for the components and final products that were used in the fusion peptide immunization. The sequence and names of the CTL epitopes can be found in Table 1. Details of fusion peptides and structure can be found in prior publications (Daftarian et al., 2005;La Rosa et al., 2002).
X=cyclohexyl-alanine.
MW= molecular weight confirmed by mass spectroscopy. Active CpG motif underlined.