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. Author manuscript; available in PMC: 2009 Oct 9.
Published in final edited form as: Nature. 2009 Mar 1;458(7239):771–775. doi: 10.1038/nature07864

Figure 3. Reprogramming cassette excision and pluripotency of the non-viral iPS cells.

Figure 3

a. Many of the undifferentiated colonies at 5 days post Cre-transfection differentiated by day 9 in the absence of an Fgf receptor inhibitor, PD173074 (bottom). b. Percentage of reprogramming cassette-free undifferentiated colonies in the absence (−PD) and presence (+PD) of PD173074. Numbers of monitored reprogramming cassette-excised colonies are indicated in parentheses. Experiments were performed in three cell lines, imO2, imO7 and TNGimO5. c. Undifferentiated cells in imO7 teratoma (left) and various tissues in imO7c8 teratoma (the other three panels). d. imO3Ec5 derived chimeric embryos (green) at 10.5 dpc (left panels). e. Transversal sections at 9.5 dpc (upper six panels) and genital ridge at 12.5 dpc (bottom panels). imO3Ec5 contributed to ectoderm (neural tissue; n with magnification), mesoderm (heart; h), endoderm (gut; g with magnification) and germ cells (red staining with anti-Oct4 antibody). f. An adult imO7c8 derived chimeric mouse.