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. Author manuscript; available in PMC: 2009 Apr 12.
Published in final edited form as: Chromosome Res. 2009 Jan 27;17(1):47–64. doi: 10.1007/s10577-008-9005-y

Fig. 7.

Fig. 7

Trichostatin A inhibits histone deacetylation, nuclear condensation and nuclear extrusion. (A) Total nuclear proteins were separated on SDS-PAGE and detected by Western blotting with antibodies against acetylated histone H4(K12). FVA erythroblasts were cultured for 24 or 48 h with erythropoietin (lanes 1 and 2) or additionally treated for the final 24 h with 100 (3) or 200 (4) nM TSA and then nuclei were isolated. The bottom panel shows histone loading controls stained with Coomassie R250. (B) Histogram of percent erythroblasts, reticulocytes and expelled nuclei in untreated cultures and in cells cultured for 24 h and then treated for the final 24 h with 100 nM TSA. (C) Examples of cytospin preparations at 44 h of untreated, control erythroblasts and erythroblasts treated with 100 nM TSA. Scale bar, 5 μm. (D) Histogram showing nuclear diameter distribution in cytospin preparations of control and TSA-treated cells at 44 h.