Figure 1.
HAT activity from BJAB lysates binds specifically to the EBNA2 and VP16 acidic domains. The wild-type EBNA2 (E2) or VP16 (VP) or their null point mutant (E2m and VPm) acidic domains were fused to GST (G), and glutathione-Sepharose beads coated with fusion protein were used in pull downs with extracts from BJAB B cell lymphoma cells. The presence of HAT activity in proteins bound to beads was determined by liquid HAT assay. HAT activity was shown as 103 × count per minute. Results from one representative experiment of five similar experiments are shown.