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The EBNA2 and VP16 acidic domains specifically bind p300, CBP, and PCAF. BJAB cell extracts were incubated with glutathione-Sepharose beads loaded with wild-type GST-EBNA2 or GST-VP16 acidic domains or with the null point mutants. The amount of p300, CBP, or PCAF that bound to the beads was assayed by SDS/PAGE followed by Western blot with anti-CBP/p300 antibody or with anti-PCAF antibody.
