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P300 coactivates LMP1 expression mediated by EBNA2 in Akata EBV-infected but EBNA2-negative cells, and PCAF potentiates the activation. Akata cells were transfected with 10 μg of SG5-EBNA2, 10 μg of SG5-p300, 20 μg of pCI-PCAF, or 20 μg of HAT-negative PCAF mutants Δ579–608 and Δ609–624 protein expression vectors. Empty vector was used to balance the transfections to 40 μg of total DNA when necessary. Whole-cell extracts were Western-blotted with the S12 anti-LMP1 mAb.
