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. 2009 Apr 22;4(4):e5267. doi: 10.1371/journal.pone.0005267

Figure 3. Sumoylation of Rpb1 does not affect its degradation in response to UV radiation.

Figure 3

Whole cell extracts were prepared from the cells that had been incubated for different times following UV irradiation. Rpb1 in the whole cell extracts were probed with antibody 8WG16 on the Western blots. Tubulin serves as an internal loading control. (A) Levels of Rpb1 in isogenic wild type (BY4741) and siz1 (strain 4245) cells. (B) Levels of wild type and K1487R mutant Rpb1 expressed in isogenic cells (CX84 and CX79). (C) Levels of Rpb1 in wild type (Y452) and hex3 slx8 (MHY3861) cells. (D) Levels of Rpb1 in isogenic cells expressing wild type (JD74-13c) or K11,15,19R mutant Smt3 (YKU116). WT, wild type.