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. 2009 Apr 22;4(4):e5267. doi: 10.1371/journal.pone.0005267

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Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Rpb1 was immunoprecipitated from the unirradiated (control, BJ5465) and UV irradiated isogenic wild type [BJ5465 (lane 2) and Y452 (lane 4)], elc1 (CR105) and def1 (SL128) cells and cells expressing wild type [CX84 (lane 6)], K330R (CR191) or K695R (CR192) mutant Rpb1 using antibody 8WG16 and probed with anti-SUMO and 8WG16 antibodies.