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. 2009 Apr 22;4(4):e5281. doi: 10.1371/journal.pone.0005281

Figure 1. Expression and purification of recombinant HcDppIVA in Pichia pastoris.

Figure 1

(A) Gly-pro-AMC zymogram of supernatants from P. pastoris transformed with empty vector and HcDppIVA-expressing Pichia (mcr#1). Molecular weights (kDa) are indicated on the right. (B) SDS-PAGE gel of supernatants from a non-expressing strain (vector) and mcr#1 as well as the purified fraction of HcDppIVA (rDppIV). The arrow indicates the band likely corresponding to the enzyme. The molecular weight markers (MWM) are in the first lane of the gel. The gel was stained with Coomassie Brilliant Blue.